ValRS Polyclonal antibody

ValRS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15931-1-AP

Synonyms

G7A, Protein G7a, Valine tRNA ligase, ValRS, Valyl-tRNA synthetase, VARS, VARS1, VARS2

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Validation Data Gallery

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  WB analysis of HL-60 using 15931-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15931-1-AP (ValRS antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 15931-1-AP

  IP result of anti-ValRS (IP:15931-1-AP, 4ug; Detection:15931-1-AP 1:500) with HL-60 cells lysate 3040ug.

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  IHC staining of human tonsillitis using 15931-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15931-1-AP (ValRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15931-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15931-1-AP (ValRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 2 publications below

Product Information

15931-1-AP targets ValRS in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ValRS fusion protein Ag8778
• Full Name: valyl-tRNA synthetase
• Calculated Molecular Weight: 1264 aa, 140 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC012808
• Gene Symbol: VARS
• Gene ID (NCBI): 7407
• RRID: AB_2213252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	Autoimmun Rev; Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase; Authors - Yoshinao Muro
human	WB,IP	Ann Rheum Dis; Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies; Authors - Jean-Baptiste Vulsteke