VASH2 Polyclonal antibody

VASH2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13303-1-AP

Synonyms

RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein

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Validation Data Gallery

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  WB analysis using 13303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13303-1-AP (VASH2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 13303-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13303-1-AP (VASH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 13303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 13303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using VASH2 antibody (13303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HUVEC cells, L02 cells, PC-12 cells
• Positive IHC detected in: human stomach cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13303-1-AP targets VASH2 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VASH2 fusion protein Ag4121
• Full Name: vasohibin 2
• Calculated Molecular Weight: 355 aa, 40 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC028194
• Gene Symbol: VASH2
• Gene ID (NCBI): 79805
• RRID: AB_10642836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. This antibody recognizes both the nuclear and cytoplasmic isoforms.(PMID:23615928; 19204325; 31235911; 26177649)