Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
17714-1-AP targets VASN in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12040 Product name: Recombinant human VASN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-349 aa of BC068575 Sequence: GCQCSQPQTVFCTARQGTTVPRDVPPDTVGLYVFENGITMLDAGSFAGLPGLQLLDLSQNQIASLPSGVFQPLANLSNLDLTANRLHEITNETFRGLRRLERLYLGKNRIRHIQPGAFDTLDRLLELKLQDNELRALPPLRLPRLLLLDLSHNSLLALEPGILDTANVEALRLAGLGLQQLDEGLFSRLRNLHDLDVSDNQLERVPPVIRGLRGLTRLRLAGNTRIAQLRPEDLAGLAALQELDVSNLSLQALPGDLSGLFPRLRLLAAARNPFNCVCPLSWFGPWVRESHVTLASPEETRCHFPPKNAGRLLLELDYADFGC Predict reactive species |
| Full Name | vasorin |
| Calculated Molecular Weight | 673 aa, 72 kDa |
| Observed Molecular Weight | 50-60 kDa, 75 kDa, 100 kDa |
| GenBank Accession Number | BC068575 |
| Gene Symbol | VASN |
| Gene ID (NCBI) | 114990 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6EMK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vasorin (VASN) is a single transmembrane type I membrane protein, also known as Slit-like 2 protein (SLITL2). It is mainly expressed in vascular smooth muscle cells and participates in many pathophysiological processes. VASN contains an EGF-like domain, ten leucine-rich repeats (LRR) and a transmembrane domain. VASN is considered as a negative regulator of transforming growth factor -\(\beta \) (TGF-\(\beta \)) signal. It may be involved in regulating cell growth, differentiation and epithelial-mesenchymal transition (EMT) by binding and regulating the activity of TGF-\(\beta \). In blood vessels, VASN is expressed in vascular smooth muscle cells, which has the characteristics of protecting blood vessels and can negatively regulate cell migration and proliferation caused by vascular injury. VASN is also involved in the reaction of cells to hypoxia and redox state, and plays a certain role in alleviating cell stress damage. Because of its high expression in specific tissues (such as blood vessels and some solid tumors), VASN is often used as a molecular marker to study vascular diseases or pathological changes of tumors. The protein has multiple bands, intact vasorin (∼100 kDa) and a soluble fragment (∼75 kDa),Cleaved frament (~50-60 kDa) (PMID: 29930755).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VASN antibody 17714-1-AP | Download protocol |
| WB protocol for VASN antibody 17714-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
