VASP Recombinant monoclonal antibody

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• VASP Recombinant monoclonal antibody (87104-3-RR)

• 

  WB analysis using 87104-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87104-3-RR (Phospho-VASP(Ser239) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

VASP Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87104-3-RR

Clone No.252207G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Vasodilator-stimulated phosphoprotein

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87104-3-RR targets VASP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: vasodilator-stimulated phosphoprotein
• Calculated Molecular Weight: 380 aa, 40 kDa
• Observed Molecular Weight: ~ 50 kDa
• GenBank Accession Number: BC038224
• Gene Symbol: VASP
• Gene ID (NCBI): 7408
• RRID: AB_3745341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50552
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition.