VAT1 Monoclonal antibody

• 

  WB analysis of HepG2 using 67474-1-Ig

  WB result of VAT1 antibody (67474-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAT1 transfected HepG2 cells.

• 

  WB analysis using 67474-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67474-1-Ig (VAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 67474-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67474-1-Ig (VAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

VAT1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67474-1-Ig

Clone No.2C8A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

VATI, Synaptic vesicle membrane protein VAT-1 homolog, EC:1.-.-.-, 2C8A7

---

Tested Applications

• Positive WB detected in: HSC-T6 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67474-1-Ig targets VAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29812

  Product name: Recombinant human VAT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-393 aa of BC015041

  Sequence: MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN

• Full Name: vesicle amine transport protein 1 homolog (T. californica)
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-50 kDa
• GenBank Accession Number: BC015041
• Gene Symbol: VAT1
• Gene ID (NCBI): 10493
• RRID: AB_2882701
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q99536
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.