VCAM-1/CD106 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis of HUVEC using 84249-4-RR (same clone as 84249-4-PBS)

  TNF alpha treated HUVEC cells were subjected to SDS PAGE followed by western blot with 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01145-3

  Sandwich ELISA standard curve of MP01145-3, Human VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 84249-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02345. 84249-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  Sample test of MP01145-3

  Serum of six individual healthy human donors was measured. The human VCAM-1/CD106 concentration of detected samples was determined to be 188.79 ng/mL with a range of 126.68 - 336.06 ng/mL.

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  IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VCAM-1/CD106 antibody (84249-4-RR, Clone: 241284B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VCAM-1/CD106 antibody (84249-4-RR, Clone: 241284B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01145-3

  Cytometric bead array standard curve of MP01145-3, VCAM-1/CD106 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84249-4-PBS. Detection antibody: 84249-2-PBS. Standard: RP02345. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 84249-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84249-4-RR against Human VCAM-1/CD106 were performed. The affinity constant is 2.18 nM.

VCAM-1/CD106 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84249-4-PBS

Clone No.241284B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

VCAM1, VCAM-1, 241284B2, CD106, INCAM 100

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Product Information

84249-4-PBS targets VCAM-1/CD106 as part of a matched antibody pair:

MP01145-3: 84249-4-PBS capture and 84249-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: vascular cell adhesion molecule 1
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001078.4
• Gene Symbol: VCAM-1
• Gene ID (NCBI): 7412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19320-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).