VDAC1/2/3 Polyclonal antibody

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  WB analysis using 11663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11663-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:50 (under 40x lens).

VDAC1/2/3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11663-1-AP

Publications(33)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

VDAC2, VDAC-2, VDAC 2, hVDAC2, FLJ23841

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, mouse heart tissue, rat brain tissue, rat heart tissue
• Positive IHC detected in: human prostate cancer tissue, human normal colon, human prostate tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 27 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

11663-1-AP targets VDAC1/2/3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VDAC1/2/3 fusion protein Ag2266
• Full Name: voltage-dependent anion channel 2
• Calculated Molecular Weight: 294 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000165
• Gene Symbol: VDAC2
• Gene ID (NCBI): 7417
• RRID: AB_2304144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P45880
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and expecially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue.(PMID: 22020053)

Publications

Species	Application	Title
human,mouse	WB	Mol Cell; MLKL activity requires a splicing-regulated, druggable intramolecular interaction; Authors - Uris Ros
mouse	WB	Nat Commun; Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis.; Authors - Shintaro Mise
mouse	IP	Autophagy; MYBL2 guides autophagy suppressor VDAC2 in the developing ovary to inhibit autophagy through a complex of VDAC2-BECN1-BCL2L1 in mammals.; Authors - Jia Yuan
human,mouse	WB,IF	Cell Death Differ; SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy.; Authors - Ying Zhang
mouse	WB	Proc Natl Acad Sci U S A; SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.; Authors - Haruhiko Miyata
rat	WB,CoIP	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jun (Verified Customer) (06-12-2022)

Works very well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

VDAC1/2/3 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11663-1-AP)