VDAC1/2/3 Polyclonal antibody

VDAC1/2/3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11663-1-AP

Synonyms

hVDAC2, VDAC 2, VDAC2



Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IHC detected inhuman prostate cancer tissue, human prostate tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11663-1-AP targets VDAC1/2/3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VDAC1/2/3 fusion protein Ag2266
Full Name voltage-dependent anion channel 2
Calculated Molecular Weight 294 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000165
Gene Symbol VDAC2
Gene ID (NCBI) 7417
RRIDAB_2304144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and expecially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue.(PMID: 22020053)

Protocols

Product Specific Protocols
WB protocol for VDAC1/2/3 antibody 11663-1-APDownload protocol
IHC protocol for VDAC1/2/3 antibody 11663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis.

Authors - Shintaro Mise
mouseIP

Autophagy

MYBL2 guides autophagy suppressor VDAC2 in the developing ovary to inhibit autophagy through a complex of VDAC2-BECN1-BCL2L1 in mammals.

Authors - Jia Yuan
human,mouseWB,IF

Cell Death Differ

SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy.

Authors - Ying Zhang
mouseWB

Proc Natl Acad Sci U S A

SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.

Authors - Haruhiko Miyata
ratWB,CoIP

Cell Death Dis

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats

Authors - Yajie Wang
humanWB

EMBO Mol Med

Conformational change of adenine nucleotide translocase-1 mediates cisplatin resistance induced by EBV-LMP1.

Authors - Lin Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jun (Verified Customer) (06-12-2022)

Works very well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
VDAC1/2/3 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11663-1-AP)