Tested Applications
| Positive WB detected in | A431 cells, HEK-293T cells, mouse testis tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55260-1-AP targets VDAC3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | voltage-dependent anion channel 3 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | NM_005662 |
| Gene Symbol | VDAC3 |
| Gene ID (NCBI) | 7419 |
| RRID | AB_10973676 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y277 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VDAC3 antibody 55260-1-AP | Download protocol |
| WB protocol for VDAC3 antibody 55260-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VDAC3 Polyclonal antibody (catalog number 55260-1-AP) has 33 citations. These appear in journals including Signal Transduction and Targeted Therapy, Cancer Discovery, Nature Cell Biology, Nature Communications, Molecular Cell, EMBO Journal, Cell Reports, Oncogene, Free Radical Biology and Medicine, Journal of Biological Chemistry, and International Journal of Molecular Sciences. Research fields span cancer biology, ferroptosis, mitochondrial biology, apoptosis, autophagy/mitophagy, cardiovascular disease, and neuroprotection, with applications mainly in Western blot, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer. | ||
Cancer Discov BRCA1-mediated dual regulation of ferroptosis exposes a vulnerability to GPX4 and PARP co-inhibition in BRCA1-deficient cancers
| ||
Nat Cell Biol De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence
| ||
Nat Commun Cleavage of CAD by caspase-3 determines the cancer cell fate during chemotherapy | ||
