VDAC3 Polyclonal antibody

VDAC3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 55260-1-AP

Synonyms

HD VDAC3, hVDAC3, VDAC 3, VDAC3

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Validation Data Gallery

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  WB analysis of A431 using 55260-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55260-1-AP (VDAC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 55260-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55260-1-AP (VDAC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 55260-1-AP

  IP Result of anti-VDAC3 (IP:55260-1-AP, 4ug; Detection:55260-1-AP 1:300) with mouse testis tissue lysate 4800ug.

• 

  IHC staining of human testis using 55260-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55260-1-AP (VDAC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55260-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55260-1-AP (VDAC3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Product Information

55260-1-AP targets VDAC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: voltage-dependent anion channel 3
• Calculated molecular weight: 31 kDa
• Observed molecular weight: 31 kDa
• GenBank accession number: NM_005662
• Gene symbol: VDAC3
• Gene ID (NCBI): 7419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.

Publications

Species	Application	Title
human	WB,IP	Front Cell Dev Biol; Autophagy Regulates VDAC3 Ubiquitination by FBXW7 to Promote Erastin-Induced Ferroptosis in Acute Lymphoblastic Leukemia.; Authors - Ting Zhu
human	WB,IHC,IP	Int J Biol Sci; The lncRNA BDNF-AS/WDR5/FBXW7 axis mediates ferroptosis in gastric cancer peritoneal metastasis by regulating VDAC3 ubiquitination.; Authors - Guoquan Huang
human	WB	Toxins (Basel); Proteomic Studies of the Mechanism of Cytotoxicity, Induced by Palytoxin on HaCaT Cells.; Authors - Dingyuan Cheng
mouse	WB	BMC Complement Med Ther; Prevotella histicola suppresses ferroptosis to mitigate ethanol-induced gastric mucosal lesions in mice; Authors - Sisi Wang

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