VE-cadherin/CD144 Polyclonal antibody

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• VE-cadherin/CD144 Polyclonal antibody (32332-1-AP)

VE-cadherin/CD144 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 32332-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

VE-cadherin, 7B4, cadherin 5, Cd144, Cdh5

• 

  WB analysis using 32332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32332-1-AP (VE-cadherin/CD144 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of bEnd.3 using 32332-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed bEnd.3 cells using VE-cadherin/CD144 antibody (32332-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: bEnd.3 cells, mouse lung tissue, rat lung tissue
• Positive IF/ICC detected in: bEnd.3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32332-1-AP targets VE-cadherin/CD144 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0921

  Product name: Recombinant Mouse VE-cadherin/CD144 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 46-592 aa of NM_009868.4

  Sequence: DWIWNQMHIDEEKNESLPHYVGKIKSNVNRQNAKYVLQGEFAGKIFGVDANTGNVLAYERLDREKVSEYFLTALIVDKNTNKNLEQPSSFTVKVHDINDNWPVFSHQVFNASVPEMSAIGTSVIRVTAVDADDPTVAGHATVLYQIVKGNEYFSIDNSGLIFTKIKNLDREKQAEYKIVVETQDALGLRGESGTATVMIRLEDINDNFPVFTQSTYTFSVPEDIRVGKPLGFLTVVDPDEPQNRMTKYSIMQGEYRDTFTIETDPKRNEGIIKPTKSLDYEVIQQYTFYIEATDPTIRYEYLSSTSGKNKAMVTINVLDVDEPPVFQRHFYHFKLPENQKKPLIGTVVAKDPDKAQRSIGYSIRKTSDRGQFFRITKQGNIYNEKELDRETYAWYNLTVEANELDSRGNPVGKESIVQVYIEVLDENDNPPEFAQPYEPKVCENAAQGKLVVQISATDKDVVPVNPKFKFALKNEDSNFTLINNHDNTANITVKYGQFNREHAKFHYLPVLISDNGVPSLTGTSTLTVGVCKCNEQGEFTFCEEMAA

• Full Name: cadherin 5
• Calculated Molecular Weight: 88kDa
• Observed Molecular Weight: 130 kDa, 100 kDa
• GenBank Accession Number: NM_009868.4
• Gene Symbol: Cdh5
• Gene ID (NCBI): 12562
• RRID: AB_3742578
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P55284
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

Publications

What published studies show

The VE-cadherin/CD144 Polyclonal antibody (Cat# 32332-1-AP) has a total of 2 citations. These appear in Phytomedicine and Frontiers in Nutrition. The research fields covered include vascular inflammation and sepsis shock (studying diacerein's protective effects on endothelial permeability), and cavernous endothelial remodeling related to bisphenol A-induced erectile dysfunction. Both studies utilized this antibody for WB and/or IF applications in mouse models.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB, IF	Phytomedicine; Diacerein attenuates sepsis shock mortality through combined suppression of vascular inflammation, stiffness, and permeability.; Authors - Minghao Luo
		Front Nutr; FN1-associated cavernous endothelial remodeling induced by bisphenol A: relevance to erectile dysfunction and attenuation by baicalin.; Authors - Xinyao Zhu