VEGFB Recombinant antibody

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  WB analysis of Transfected HEK-293T using 84734-3-RR

  Human VEGFB Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 84734-3-RR (VEGFB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84734-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84734-3-RR against Human VEGFB were performed. The affinity constant is 4.28 nM.

VEGFB Recombinant Antibody for WB, ELISA

Cat No. 84734-3-RR

Clone No.242024C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

VRF, VEGF-related factor, VEGF-B, VEGF related factor, VEGF B

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Tested Applications

• Positive WB detected in: Human VEGFB Transfected HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84734-3-RR targets VEGFB in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: vascular endothelial growth factor B
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22-30 kDa
• GenBank Accession Number: BC008818
• Gene Symbol: VEGFB
• Gene ID (NCBI): 7423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P49765
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vascular endothelial growth factor B (VEGFB) is the third vascular endothelial growth factor family member to be discovered in 1996 . It has been demonstrated that vascular endothelial growth factor B (VEGFB) plays a vital role in regulating vascular biological function. VEGFB binds to vascular endothelial growth factor receptor 1 (VEGFR1) and neuropilin 1 (NRP1), which activates a lot of downstream activators similar to most tyrosine kinase receptors such as p38 MAPK, ERK/MAPK, PKB/AKT, and PI3K.(PMID: 34948148, PMID: 37375853)