VEGFD Polyclonal antibody

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• VEGFD Polyclonal antibody (30323-1-AP)

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  WB analysis using 30323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30323-1-AP (VEGFD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 30323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30323-1-AP (VEGFD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 30323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30323-1-AP (VEGFD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VEGFD Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30323-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Figf, c fos induced growth factor, Vascular endothelial growth factor D, VEGF D, VEGF-D

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30323-1-AP targets VEGFD in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: c-fos induced growth factor
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: NM_010216
• Gene Symbol: Figf
• Gene ID (NCBI): 14205
• RRID: AB_3742335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P97946
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VEGFD is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.