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Anti-Rat VEGFR1/Flt-1 Rabbit Recombinant Antibody, PBS Only (98418-2-PBS)

Anti-Rat VEGFR1/Flt-1 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Rat VEGFR1/Flt-1 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98418-2-PBS-1MG

VEGFR1/Flt-1 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98418-2-PBS

Clone No. 242311D5

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Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC (Intra)

Synonyms

Flt1, FLT-1, VEGFR-1, VGFR1

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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Filter: AllFC (Intra)Affinity

FC experiment of Transfected HEK-293T using 98418-2-RR (same clone as 98418-2-PBS)
1x10^6 HEK-293T cells or VEGFR1/Flt-1 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat VEGFR1/Flt-1 Rabbit RecAb (98418-2-RR, Clone:242311D5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98418-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 HEK-293T cells or VEGFR1/Flt-1 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat VEGFR1/Flt-1 Rabbit RecAb (98418-2-RR, Clone:242311D5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98418-2-PBS in a different storage buffer formulation.
FC experiment of Transfected HEK-293T using 98418-2-RR (same clone as 98418-2-PBS)
1x10^6 HEK-293T cells or VEGFR1/Flt-1 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat VEGFR1/Flt-1 Rabbit RecAb (98418-2-RR, Clone: 242311D5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98418-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 HEK-293T cells or VEGFR1/Flt-1 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat VEGFR1/Flt-1 Rabbit RecAb (98418-2-RR, Clone: 242311D5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98418-2-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98418-2-RR
Biolayer interferometry (BLl) kinetic assays of 98418-2-RR against Rat VEGFR1/Flt-1 were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

98418-2-PBS targets VEGFR1/Flt-1 in FC (Intra) applications and shows reactivity with rat samples.

Tested Reactivity	rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1167 Product name: Recombinant Rat VEGFR1/Flt-1 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 23-758 aa of Sequence: YCSGSKLKGPELSLKGTQHVMQAGQTLFLKCRGEAAHSWSLPTTVSQEDKKLSVTRSACGRNNRQFCSTLTLNMAQANHTGLYSCRYLPKSTSKEKKMESAIYIFVSDAGSPFIEMHSDIPKLVHMTEGRELIIPCRVTSPNITVTLKKFPFDALTPDGQRIAWDSRRGFIIANATYKEIGLLTCEATVNGHLYQTSYLTHRQTNTILDVQISPPSPVRFLRGQTLVLNCTVTTDLNTRVQMSWNYPGKATKRASIRQRIDQSNPHSNVFHSVLKINNVESRDKGLYTCRVKSGSSFRTFNTSVHVYEKGFISVKHRKQQVQETIAGKRSHRLSMKVKAFPSPEVVWLKDGVPATEKSARYSVHGYSLIIKDVTAEDAGDYTILLGIKQSKLFRNLTATLIVNVKPQIYEKSVSSLPSPPLYPLGSRQVLTCTVYGIPQPTIKWLWHPCHYNHSKERNDFCFGSEESFILDSSSNIGNRIEGITQRMMVIEGTNKTVSTLVVADSRTPGSYSCKAFNKIGTVERDIRFYVTDVPNGFHVSLEKIPTEGEDLKLSCVVSKFLYRDITWILLRTVNNRTMHHSISKQKMATTQDYSITLNLVIKNVSLEDSGTYACRARNIYTGEEILRKTEVLVRDLEAPLLLQNLSDHEVSISGSTTLDCQARGVPAPQITWFKNNHKIQQEPGIILGPGNSTLFIERVTEEDEGVYRCRATNQKGVVESSAYLTVQGTSDKSNLE Predict reactive species
Full Name	fms-related tyrosine kinase 1
Gene Symbol	Flt1
Gene ID (NCBI)	54251
RRID	AB_3746059
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P53767
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders, and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF, and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion.