VEGFR2/KDR Recombinant antibody

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  WB analysis of mouse lung using 83366-1-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83366-1-RR (VEGFR2/KDR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83366-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83366-1-RR against Mouse VEGFR2/KDR were performed. The affinity constant is 1.88 nM.

VEGFR2/KDR {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83366-1-RR

Clone No.240214D7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Kdr, Krd 1, Flk1, Flk 1, 6130401C07

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Tested Applications

• Positive WB detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83366-1-RR targets VEGFR2/KDR in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: kinase insert domain protein receptor
• Calculated Molecular Weight: 150KD
• Observed Molecular Weight: 150-190 kDa
• GenBank Accession Number: NM_010612.2
• Gene Symbol: VEGFR-2
• Gene ID (NCBI): 16542
• RRID: AB_3671020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35918-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VEGFR2, also named CD309, KDR, and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In the case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis, and sprouting.