|Positive WB detected in||Jurkat cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells, rat brain tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10124-2-AP targets VGLL1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VGLL1 fusion protein Ag0169|
|Full Name||vestigial like 1 (Drosophila)|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||26-29 kDa|
|GenBank accession number||BC000045|
|Gene ID (NCBI)||51442|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VGLL1 is a specific coactivator for the mammalian TEFs. It can bind proteins of the TEA domain family of transcription factors. Vgll1-TEFs complex upregulates the expression of IGFBP-5, a proliferation-promoting gene, and facilitates anchorage-independent cell proliferation
PI3K/AKT/β-Catenin Signaling Regulates Vestigial-Like 1 Which Predicts Poor Prognosis and Enhances Malignant Phenotype in Gastric Cancer.
Endocr Relat Cancer
Vgll1 expression is associated with a triple-negative basal-like phenotype in breast cancer.