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VGLL1 Polyclonal antibody

VGLL1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10124-2-AP

Synonyms

Protein TONDU, TDU, vestigial like 1 (Drosophila), Vgl 1, VGL1, VGLL1



Tested Applications

Positive WB detected inJurkat cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10124-2-AP targets VGLL1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VGLL1 fusion protein Ag0169
Full Name vestigial like 1 (Drosophila)
Calculated molecular weight 29 kDa
Observed molecular weight 26-29 kDa
GenBank accession numberBC000045
Gene symbol VGLL1
Gene ID (NCBI) 51442
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VGLL1 is a specific coactivator for the mammalian TEFs. It can bind proteins of the TEA domain family of transcription factors. Vgll1-TEFs complex upregulates the expression of IGFBP-5, a proliferation-promoting gene, and facilitates anchorage-independent cell proliferation

Protocols

Product Specific Protocols
WB protocol for VGLL1 antibody 10124-2-APDownload protocol
IHC protocol for VGLL1 antibody 10124-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancers (Basel)

PI3K/AKT/β-Catenin Signaling Regulates Vestigial-Like 1 Which Predicts Poor Prognosis and Enhances Malignant Phenotype in Gastric Cancer.

Authors - Bo-Kyung Kim
  • KD Validated

Endocr Relat Cancer

Vgll1 expression is associated with a triple-negative basal-like phenotype in breast cancer.

Authors - María Ángeles Castilla