|Positive IHC detected in||human liver cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16538-1-AP targets VHL in IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||VHL fusion protein Ag9843|
|Full Name||von Hippel-Lindau tumor suppressor|
|Calculated molecular weight||172 aa, 20 kDa|
|GenBank accession number||BC058831|
|Gene ID (NCBI)||7428|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jingwen (Verified Customer) (01-27-2020)
This antibody worked very well on the cancer samples by performing western blot and immunofluorescence.
Jie (Verified Customer) (01-27-2020)
achieved positive staining in liver cancer cells
Lunfeng (Verified Customer) (01-27-2020)