VHL Polyclonal antibody

VHL Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16538-1-AP

Synonyms

HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1, Von Hippe-Lindau



Tested Applications

Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16538-1-AP targets VHL in IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VHL fusion protein Ag9843
Full Name von Hippel-Lindau tumor suppressor
Calculated molecular weight 172 aa, 20 kDa
GenBank accession numberBC058831
Gene symbol VHL
Gene ID (NCBI) 7428
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.

Protocols

Product Specific Protocols
IHC protocol for VHL antibody 16538-1-APDownload protocol
IF protocol for VHL antibody 16538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer.

Authors - Fabao Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jingwen (Verified Customer) (01-27-2020)

This antibody worked very well on the cancer samples by performing western blot and immunofluorescence.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
  • Cell Tissue Type: Cancer tissue
FH

Jie (Verified Customer) (01-27-2020)

achieved positive staining in liver cancer cells

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:200
FH

Lunfeng (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,