Villin like Polyclonal antibody

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  WB analysis using 15130-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15130-1-AP (VILL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 15130-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15130-1-AP (VILL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 15130-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat small intestine using 15130-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Villin like Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 15130-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

VILL, Villin-like protein, Villin like protein

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Tested Applications

• Positive WB detected in: 37°C incubated mouse colon tissue, rat colon
• Positive IHC detected in: mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse small intestine tissue, rat small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

15130-1-AP targets Villin like in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7259

  Product name: Recombinant human VILL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 334-687 aa of BC000243

  Sequence: SASTTRLFQVQGTDSHNTRTMEVPARASSLNSSDIFLLVTASVCYLWFGKGCNGDQREMARVVVTVISRKNEETVLEGQEPPHFWEALGGRAPYPSNKRLPEEVPSFQPRLFECSSHMGCLVLAEVGFFSQEDLDKYDIMLLDTWQEIFLWLGEAASEWKEAVAWGQEYLKTHPAGRSPATPIVLVKQGHEPPTFIGWFFTWDPYKWTSHPSHKEVVDGSPAAASTISEITAEVNNLRLSRWPGNGRAGAVALQALKGSQDSSENDLVRSPKSAGSRTSSSVSSTSATINGGLRREQLMHQAVEDLPEGVDPARREFYLSDSDFQDIFGKSKEEFYSMATWRQRQEKKQLGFF

• Full Name: villin-like
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 96-100 kDa
• GenBank Accession Number: BC000243
• Gene Symbol: VILL
• Gene ID (NCBI): 50853
• RRID: AB_2918038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15195
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	ACS Appl Bio Mater; Fullerenol Doped Matrigel Promotes Maturation of Intestinal Organoids by Inhibiting Oxidative Stress and Apoptosis; Authors - Fengkai Ruan