VIM Monoclonal antibody

VIM Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 65039-1-Ig

Synonyms



Tested Applications

Positive WB detected inA549 cells, A549
Positive IHC detected inLung cancer tissue, human lung cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

65039-1-Ig targets VIM in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name vimentin
Calculated molecular weight 54 kDa
GenBank accession numberBC066956
Gene symbol VIM
Gene ID (NCBI) 7431
Conjugate Unconjugated
Form Liquid
Purification Method Other
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at 2-8°C.

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

High expression of hnRNPA1 promotes cell invasion by inducing EMT in gastric cancer.

Authors - Yahua Chen