Vimentin Monoclonal antibody

VIM Monoclonal Antibody for WB, IHC, ELISA
Cat No. 65039-1-Ig

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

VIM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, A549
Positive IHC detected inLung cancer tissue, human lung cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

65039-1-Ig targets VIM in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein Predict reactive species
Full Name vimentin
Calculated Molecular Weight 54 kDa
GenBank Accession NumberBC066956
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
Conjugate Unconjugated
Form Liquid
Purification MethodOther
UNIPROT IDP08670
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Publications

SpeciesApplicationTitle
HumanWB

Oncol Rep

High expression of hnRNPA1 promotes cell invasion by inducing EMT in gastric cancer.

Authors - Yahua Chen
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