VIRMA/KIAA1429 Polyclonal antibody, PBS Only

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• VIRMA/KIAA1429 Polyclonal antibody, PBS Only (30166-1-PBS)

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  WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 30166-1-AP (same clone as 30166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 30166-1-AP (same clone as 30166-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using VIRMA/KIAA1429 antibody (30166-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

VIRMA/KIAA1429 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 30166-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA1429, VIRMA, MSTP054, virilizer

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Product Information

30166-1-PBS targets VIRMA/KIAA1429 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32830

  Product name: Recombinant human KIAA1429 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 786-1086 aa of BC003701

  Sequence: FMRQILNSDTIGCCGDDNGLMEVEGAHTSRTMSINAAELKQLLQSKEESPENLFLELEKLVLEHSKDDDNLDSLLDSVVGLKQMLESSGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFTPFQAEEIDTDLDLVKVDLIELSEKCCSDFDLHSELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHVVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGIPPPKRPLKVSQKISSRGGFSGNRGGRGAF

• Full Name: KIAA1429
• Observed Molecular Weight: 200-210 kDa
• GenBank Accession Number: BC003701
• Gene Symbol: KIAA1429
• Gene ID (NCBI): 25962
• RRID: AB_2935521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q69YN4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274). Moreover, this antibody can not recognize short isoforms.