VISTA Recombinant monoclonal antibody

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• VISTA Recombinant monoclonal antibody (83919-3-RR)

VISTA Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83919-3-RR Copied!

Clone No. 241003F4

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C10orf54, 241003F4, B7-H5, GI24, PP2135

• 

  WB analysis of HeLa using 83919-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 83919-3-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 83919-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83919-3-RR against Human VISTA were performed. The affinity constant is 6.56 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83919-3-RR targets VISTA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0017

  Product name: Recombinant Human VISTA/C10orf54 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 33-194 aa of BC020568

  Sequence: FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAA

• Full Name: chromosome 10 open reading frame 54
• Calculated Molecular Weight: 311 aa, 34 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC020568
• Gene Symbol: VISTA
• Gene ID (NCBI): 64115
• RRID: AB_3671498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H7M9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.