VISTA Recombinant antibody

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  WB analysis using 86114-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86114-3-RR (VISTA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

VISTA Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86114-3-RR

Clone No.250122E7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Dies1, PD-1H, Platelet receptor Gi24, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor

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Tested Applications

• Positive WB detected in: bEnd.3 cells, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86114-3-RR targets VISTA in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VISTA fusion protein Eg2809
• Full Name: RIKEN cDNA 4632428N05 gene
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 40-55 kDa
• GenBank Accession Number: NM_001159572.1
• Gene Symbol: 4632428N05Rik
• Gene ID (NCBI): 74048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9D659
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VISTA, also known as PD-1H, Dies1, and Gi24, is encoded by the Vsir gene in mice. VISTA is a type I transmembrane protein that acts as an immune-regulatory checkpoint on hematopoietic cells and shares homology with both the B7 family ligand PD-L1 and the CD28 family receptor PD-1. In mice, VISTA is expressed predominantly in hematopoietic tissues, such as the spleen, thymus, lymph node, and bone marrow (BM). VISTA plays a key role in regulating immune system responses and maintaining homeostasis.