VMA21 Polyclonal antibody

VMA21 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21921-1-AP

Synonyms

MEAX, VMA21, XMEA



Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, human placenta tissue, mouse thymus tissue, HepG2 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 21921-1-AP is VMA21 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VMA21 fusion protein Ag16439
Full Name VMA21 vacuolar H+-ATPase homolog (S. cerevisiae)
Calculated molecular weight 101 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC105694
Gene symbol VMA21
Gene ID (NCBI) 203547
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VMA21 antibody 21921-1-APDownload protocol
IHC protocol for VMA21 antibody 21921-1-APDownload protocol
IP protocol for VMA21 antibody 21921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Transl Med

Hsa_circ_0001361 facilitates the progress of lung adenocarcinoma cells via targeting miR-525-5p/VMA21 axis.

Authors - Hong-Yu Shen
humanWB

Nature

CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.

Authors - Chioniso P Masamha