VMA21 Polyclonal antibody

VMA21 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21921-1-AP

Synonyms

MEAX, VMA21, XMEA



Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, human placenta tissue, mouse thymus tissue, HepG2 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 21921-1-AP is VMA21 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VMA21 fusion protein Ag16439
Full Name VMA21 vacuolar H+-ATPase homolog (S. cerevisiae)
Calculated molecular weight 101 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC105694
Gene symbol VMA21
Gene ID (NCBI) 203547
RRIDAB_10896457
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VMA21 antibody 21921-1-APDownload protocol
IHC protocol for VMA21 antibody 21921-1-APDownload protocol
IP protocol for VMA21 antibody 21921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.

Authors - Chioniso P Masamha
humanWB

Autophagy

Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency

Authors - Fangyang Wang
human,mouseWB

J Transl Med

Hsa_circ_0001361 facilitates the progress of lung adenocarcinoma cells via targeting miR-525-5p/VMA21 axis.

Authors - Hong-Yu Shen