VMA21 Polyclonal antibody

VMA21 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21921-1-AP

Synonyms

MEAX, VMA21, XMEA

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Validation Data Gallery

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  WB analysis using 21921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21921-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21921-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 21921-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 21921-1-AP

  IP result of anti-VMA21 (IP:21921-1-AP, 3ug; Detection:21921-1-AP 1:1000) with MCF-7 cells lysate 4000ug.

• 

  IHC staining of mouse brain using 21921-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21921-1-AP (VMA21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21921-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21921-1-AP (VMA21 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21921-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21921-1-AP (VMA21 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, human placenta tissue, mouse thymus tissue, HepG2 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: mouse brain tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

The immunogen of 21921-1-AP is VMA21 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VMA21 fusion protein Ag16439
• Full Name: VMA21 vacuolar H+-ATPase homolog (S. cerevisiae)
• Calculated Molecular Weight: 101 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC105694
• Gene Symbol: VMA21
• Gene ID (NCBI): 203547
• RRID: AB_10896457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.; Authors - Chioniso P Masamha
human	WB	Autophagy; Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency; Authors - Fangyang Wang
human,mouse	WB	J Transl Med; Hsa_circ_0001361 facilitates the progress of lung adenocarcinoma cells via targeting miR-525-5p/VMA21 axis.; Authors - Hong-Yu Shen
mouse	WB	Autophagy; Aerobic exercise attenuates autophagy-lysosomal flux deficits by ADRB2/β2-adrenergic receptor-mediated V-ATPase assembly factor VMA21 signaling in APP-PSEN1/PS1 mice; Authors - Jia-Jun Wu