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VNN2 Polyclonal antibody

VNN2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25643-1-AP

Synonyms

FOAP 4, GPI 80, Protein FOAP 4, vanin 2, VNN2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using VNN2 Polyclonal antibody (25643-1-AP)

    WB analysis of HeLa using 25643-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25643-1-AP (VNN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VNN2 Polyclonal antibody (25643-1-AP)

    IF Staining of HeLa using 25643-1-AP

    Immunofluorescent analysis of HeLa cells using 25643-1-AP (VNN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25643-1-AP is VNN2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VNN2 fusion protein Ag22345
Full Name vanin 2
Calculated Molecular Weight 520 aa, 59 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC126145
Gene Symbol VNN2
Gene ID (NCBI) 8875
RRIDAB_2880173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VNN2 antibody 25643-1-APDownload protocol
IF protocol for VNN2 antibody 25643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncol Rep

MicroRNA‑106a regulates the proliferation and invasion of human osteosarcoma cells by targeting VNN2.

Authors - Yao Chen
View Article