Various lysates were subjected to SDS PAGE followed by western blot with 83901-5-RR (VPRBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83901-5-RR (VPRBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 83901-5-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-5-RR, Clone: 241028B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-5-RR, Clone: 241028B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of U-2 OS using 83901-5-RR
1x10^6 U-2 OS were intracellularly stained with 0.25 ug VPRBP Recombinant antibody (83901-5-RR, Clone:241028B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
1x10^6 U-2 OS were intracellularly stained with 0.25 ug VPRBP Recombinant antibody (83901-5-RR, Clone:241028B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
Affinity and Kinetic Characterization of 83901-5-RR
Biolayer interferometry (BLl) kinetic assays of 83901-5-RR against Human VPRBP were performed. The affinity constant is 44 pM.
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mouse testis tissue, human prostate cancer tissue, mouse brain tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Positive FC (Intra) detected in
U-2 OS
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
83901-5-RR targets VPRBP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507 amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).
Various lysates were subjected to SDS PAGE followed by western blot with 83901-5-RR (VPRBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human prostate cancer using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 83901-5-RR
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 83901-5-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-5-RR, Clone: 241028B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC (INTRA) Figures
FC experiment of U-2 OS using 83901-5-RR
1x10^6 U-2 OS were intracellularly stained with 0.25 ug VPRBP Recombinant antibody (83901-5-RR, Clone:241028B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
AFFINITY Figures
Affinity and Kinetic Characterization of 83901-5-RR
Biolayer interferometry (BLl) kinetic assays of 83901-5-RR against Human VPRBP were performed. The affinity constant is 44 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
VPRBP Recombinant antibody
Catalog Number
83901-5-RR
Citations
-
Dilutions
WB : 1:1000-1:6000 IHC : 1:250-1:1000 IF/ICC : 1:500-1:2000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
