Tested Applications
| Positive WB detected in | Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11915-1-AP targets VPREB3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2528 Product name: Recombinant human VPREB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-123 aa of BC020666 Sequence: MACRCLSFLLMGTFLSVSQTVLAQLDALLVFPGQVAQLSCTLSPQHVTIRDYGVSWYQQRAGSAPRYLLYYRSEEDHHRPADIPDRFSAAKDEAHNACVLTISPVQPEDDADYYCSVGYGFSP Predict reactive species |
| Full Name | pre-B lymphocyte 3 |
| Calculated Molecular Weight | 123 aa, 14 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC020666 |
| Gene Symbol | VPREB3 |
| Gene ID (NCBI) | 29802 |
| RRID | AB_2216942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKI3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VPREB3 (formerly named 8HS20) is a protein that associates with mu chains in the endoplasmic reticulum of pre-B cell lines (PMID: 8491176; 22684248). VPREB3 is normally expressed by precursor B cells in bone marrow and by a subset of normal germinal center B cells in secondary lymphoid organs. It has been reported that VPREB3 expression is associated with B-cell tumors with c-MYC abnormalities (PMID: 20823132).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPREB3 antibody 11915-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VPREB3 Polyclonal antibody (Cat# 11915-1-AP) has 1 total citation. It was cited in Life Sciences (2025), in a study investigating how methyltransferase-like 16 drives diabetic nephropathy progression through epigenetic suppression of VPREB3. The research field encompasses epigenetics and kidney disease. The antibody was used in WB and IHC applications on rat samples.
| Species | Application | Title |
|---|---|---|
Life Sci Methyltransferase-like 16 drives diabetic nephropathy progression via epigenetic suppression of V-set pre-B cell surrogate light chain 3 |
