Tested Applications
| Positive IHC detected in | human liver tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24505-1-AP targets VPS13B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21368 Product name: Recombinant human VPS13B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 64-412 aa of BC132978 Sequence: ELRIHVPWTKLGSEPVVITINTMECILKLKDGIQDDHESCGSNSTNRSTAESTKSSIKPRRMQQAAPTDPDLPPGYVQSLIRRVVNNVNIVINNLILKYVEDDIVLSVNITSAECYTVGELWDRAFMDISATDLVLRKVINFSDCTVCLDKRNASGKIEFYQDPLLYKCSFRTRLHFTYENLNSKMPSVIKIHTLVESLKLSITDQQLPMFIRIMQLGIALYYGEIGNFKEGEIEDLTCHNKDMLGNITGSEDETRIDMQYPAQHKGQELYSQQDEEQPQGWVSWAWSFVPAIVSYDDGEEDFVGNDPASTMHQQKAQTLKDPIVSIGFYCTKATVTFKVGLFSCCLYL Predict reactive species |
| Full Name | vacuolar protein sorting 13 homolog B (yeast) |
| Calculated Molecular Weight | 4022 aa, 449 kDa |
| GenBank Accession Number | BC132978 |
| Gene Symbol | VPS13B |
| Gene ID (NCBI) | 157680 |
| RRID | AB_2879579 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z7G8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VPS13 (vacuolar protein sorting 13 ) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VPS13B antibody 24505-1-AP | Download protocol |
| IHC protocol for VPS13B antibody 24505-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-VPS13B antibody (Cat# 24505-1-AP) has 4 citations published in Nature Communications, Cell & Molecular Life Sciences, Journal of Cell Biology, and bioRxiv. Research fields include Golgi structure and dynamics, membrane trafficking, acrosome biogenesis, and cortical malformation. Applications span immunofluorescence and Western blot in mouse and human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice. | ||
Cell Mol Life Sci Vps13b is required for acrosome biogenesis through functions in Golgi dynamic and membrane trafficking. | ||
J Cell Biol VPS13B is localized at the interface between Golgi cisternae and is a functional partner of FAM177A1 | ||
bioRxiv VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1 |
