VPS13B Polyclonal antibody

VPS13B Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24505-1-AP

Synonyms

CHS1, COH1, Cohen syndrome protein 1, KIAA0532, VPS13B

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Validation Data Gallery

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  IHC staining of human liver using 24505-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24505-1-AP (VPS13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 24505-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24505-1-AP (VPS13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24505-1-AP (VPS13B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 24505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24505-1-AP (VPS13B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 24505-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS13B antibody (24505-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human liver tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

24505-1-AP targets VPS13B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS13B fusion protein Ag21368
• Full Name: vacuolar protein sorting 13 homolog B (yeast)
• Calculated Molecular Weight: 4022 aa, 449 kDa
• GenBank Accession Number: BC132978
• Gene Symbol: VPS13B
• Gene ID (NCBI): 157680
• RRID: AB_2879579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS13 (vacuolar protein sorting 13 ) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

Publications

Species	Application	Title
mouse	IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu
mouse	IF	Cell Mol Life Sci; Vps13b is required for acrosome biogenesis through functions in Golgi dynamic and membrane trafficking.; Authors - Romain Da Costa
human	WB	bioRxiv; VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1; Authors - Berrak Ugur
human	WB	J Cell Biol; VPS13B is localized at the interface between Golgi cisternae and is a functional partner of FAM177A1; Authors - Berrak Ugur