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  • KD/KO Validated

VPS24 Polyclonal antibody

VPS24 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15472-1-AP

Synonyms

CGI 149, CHMP3, Chromatin modifying protein 3, hVps24, NEDF, VPS24



Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15472-1-AP targets VPS24 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS24 fusion protein Ag7776
Full Name vacuolar protein sorting 24 homolog (S. cerevisiae)
Calculated molecular weight 25 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC004419
Gene symbol VPS24
Gene ID (NCBI) 51652
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VPS24 antibody 15472-1-APDownload protocol
IHC protocol for VPS24 antibody 15472-1-APDownload protocol
IF protocol for VPS24 antibody 15472-1-APDownload protocol
IP protocol for VPS24 antibody 15472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.

Authors - Jorge Larios
  • KD Validated
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier