VPS25 Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS25 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 15669-1-AP

Synonyms

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

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Validation Data Gallery

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  WB analysis of A549 using 15669-1-AP

  WB result of VPS25 antibody (15669-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS25 transfected A549 cells.

• 

  WB analysis using 15669-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15669-1-AP

  WB result of VPS25 antibody (15669-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS25 transfected HepG2 cells.

• 

  WB analysis of HeLa using 15669-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15669-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15669-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15669-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15669-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using VPS25 antibody (15669-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, human brain tissue, mouse liver tissue, A549 cells, rat liver tissue, mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, mouse brain tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

15669-1-AP targets VPS25 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS25 fusion protein Ag8134
• Full Name: vacuolar protein sorting 25 homolog (S. cerevisiae)
• Calculated Molecular Weight: 176 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC006282
• Gene Symbol: VPS25
• Gene ID (NCBI): 84313
• RRID: AB_2215019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
human,mouse	WB,IP	Sci Adv; Lipid-anchored proteasomes control membrane protein homeostasis; Authors - Ruizhu Zhang ; KD Validated
mouse	WB	Nat Commun; Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.; Authors - Luana L Scheffer
canine	WB	Curr Biol; A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.; Authors - Eva P Karasmanis
human	WB	J Cell Biol; VPS37A directs ESCRT recruitment for phagophore closure.; Authors - Yoshinori Takahashi
pig	WB,IF	PLoS Pathog; Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.; Authors - Chun-Chun Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Viviana (Verified Customer) (04-27-2022)

It works very well for WB (1:1000, KD validated) and for IP (2 ug, endogenous protein).

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse embryonic tissue isolated from wild type and KD mutantss

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