VPS26B Polyclonal antibody

VPS26B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 15915-1-AP

Synonyms

Vesicle protein sorting 26B, Vacuolar protein sorting-associated protein 26B

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Validation Data Gallery

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  WB analysis using 15915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15915-1-AP (VPS26B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15915-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15915-1-AP (VPS26B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15915-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15915-1-AP (VPS26B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15915-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15915-1-AP (VPS26B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15915-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS26B antibody (15915-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, human brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

15915-1-AP targets VPS26B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS26B fusion protein Ag8708
• Full Name: vacuolar protein sorting 26 homolog B (S. pombe)
• Calculated Molecular Weight: 336 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC009747
• Gene Symbol: VPS26B
• Gene ID (NCBI): 112936
• RRID: AB_2215067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In mammals, there are two paralogues of yeast Vps26, VPS26A and VPS26B (PMID: 16190980). VPS26 is a component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. VPS26A and VPS26B subunits define distinct retromer complexes (PMID: 21920005). The retromer complex is important in recycling transmembrane receptors from endosomes to the trans-Golgi network (TGN).

Publications

Species	Application	Title
mouse	WB	Mol Psychiatry; Full recovery of the Alzheimer's disease phenotype by gain of function of vacuolar protein sorting 35.; Authors - Jian-Guo Li
mouse	WB	Nat Commun; Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis.; Authors - Luca Muzio
human	WB	Mol Psychiatry; VPS35 regulates tau phosphorylation and neuropathology in tauopathy.; Authors - Alana N Vagnozzi
rat	WB,CoIP,IF	Cell Death Differ; Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.; Authors - Jing Lu
human	WB, IP	Proc Natl Acad Sci U S A; A unique PDZ domain and arrestin-like fold interaction reveals mechanistic details of endocytic recycling by SNX27-retromer.; Authors - Matthew Gallon
human	WB	Ann Neurol; Dysregulation of the Retromer Complex System in Down Syndrome.; Authors - Mary Elizabeth Curtis

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xin (Verified Customer) (10-10-2022)

It is reliable to detect VPS26B in mouse brain lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

VPS26B Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:15915-1-AP)