Tested Applications
| Positive WB detected in | Jurkat cells, human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15915-1-AP targets VPS26B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8708 Product name: Recombinant human VPS26B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-336 aa of BC009747 Sequence: MSFFGFGQSVEVEILLNDAESRKRAEHKTEDGKKEKYFLFYDGETVSGKVSLALKNPNKRLEHQGIKIEFIGQIELYYDRGNHHEFVSLVKDLARPGEITQSQAFDFEFTHVEKPYESYTGQNVKLRYFLRATISRRLNDVVKEMDIVVHTLSTYPELNSSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIKHMEIDIIKRETTGTGPNVYHENDTIAKYEIMDGAPVRGESIPIRLFLAGYELTPTMRDINKKFSVRYYLNLVLIDEEERRYFKQQEVVLWRKGDIVRKSMSHQAAIASQRFEGTTSLGEVRTPSQLSDNNCRQ Predict reactive species |
| Full Name | vacuolar protein sorting 26 homolog B (S. pombe) |
| Calculated Molecular Weight | 336 aa, 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC009747 |
| Gene Symbol | VPS26B |
| Gene ID (NCBI) | 112936 |
| RRID | AB_2215067 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q4G0F5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
In mammals, there are two paralogues of yeast Vps26, VPS26A and VPS26B (PMID: 16190980). VPS26 is a component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. VPS26A and VPS26B subunits define distinct retromer complexes (PMID: 21920005). The retromer complex is important in recycling transmembrane receptors from endosomes to the trans-Golgi network (TGN).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VPS26B antibody 15915-1-AP | Download protocol |
| IHC protocol for VPS26B antibody 15915-1-AP | Download protocol |
| WB protocol for VPS26B antibody 15915-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SNX27 antibody (Cat# 15915-1-AP) has 10 citations. They appear in Nature Communications, Cell Death & Differentiation, Molecular Psychiatry, Acta Pharmacologica Sinica, PNAS, Annals of Neurology, and Journal of Cell Science. Research fields span neurodegeneration (ALS, Alzheimer's disease, tauopathy, Down syndrome), heart failure, and endocytic recycling/retromer trafficking.
| Species | Application | Title |
|---|---|---|
Nat Commun Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis. | ||
Cell Death Differ Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3. | ||
Mol Psychiatry Full recovery of the Alzheimer's disease phenotype by gain of function of vacuolar protein sorting 35. | ||
Acta Pharmacol Sin SNX3 mediates heart failure by interacting with HMGB1 and subsequently facilitating its nuclear-cytoplasmic translocation |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The reviewer used the antibody at a 1:2000 dilution on mouse brain lysate and found it reliable for detecting VPS26B. Overall, the feedback is positive, confirming the antibody's performance in WB for its target.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xin (Verified Customer) (10-10-2022) | It is reliable to detect VPS26B in mouse brain lysate.
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