VPS28 Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS28 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15478-1-AP

Synonyms

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28

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Validation Data Gallery

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  WB analysis of mouse brain using 15478-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15478-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15478-1-AP

  IP result of anti-VPS28 (IP:15478-1-AP, 4ug; Detection:15478-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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  IF Staining of HepG2 using 15478-1-AP

  Immunofluorescent analysis of HepG2 cells using 15478-1-AP (VPS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Jurkat cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Product Information

15478-1-AP targets VPS28 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS28 fusion protein Ag7784
• Full Name: vacuolar protein sorting 28 homolog (S. cerevisiae)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC006485
• Gene Symbol: VPS28
• Gene ID (NCBI): 51160
• RRID: AB_2878144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.

Publications

Species	Application	Title
human	IF	PLoS Genet; AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.; Authors - Chiara Merigliano
human	WB,IF	iScience; VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion.; Authors - Xiaohua Dong ; KD Validated
human	WB	Curr Protoc; Budget-Friendly Generation, Biochemical Analyses, and Lentiviral Transduction of Patient-Derived Colon Organoids; Authors - Emilie Rigaux
human	WB	J Extracell Biol; RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer; Authors - Yu-Chan Chang
human	WB,IF	Nat Commun; Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy; Authors - Takashi Nozawa ; KD Validated