VPS37A Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS37A Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 11870-1-AP

Synonyms

Vacuolar protein sorting-associated protein 37A, hVps37A, Hepatocellular carcinoma-related protein 1, HCRP1, ESCRT-I complex subunit VPS37A

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Validation Data Gallery

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  WB analysis using 11870-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11870-1-AP

  WB result of VPS37A antibody (11870-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS37A transfected MCF-7 cells.

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  WB analysis of human liver using 11870-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11870-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11870-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11870-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 11870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human liver using 11870-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VPS37A antibody (11870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human liver tissue, MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

11870-1-AP targets VPS37A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS37A fusion protein Ag2453
• Full Name: vacuolar protein sorting 37 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC022363
• Gene Symbol: VPS37A
• Gene ID (NCBI): 137492
• RRID: AB_2215230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

Publications

Species	Application	Title
human	WB,IF	Autophagy; Removal of hypersignaling endosomes by simaphagy; Authors - Simona M Migliano ; KO Validated
human	WB	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F
human	WB	Structure; The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.; Authors - Monica Agromayor
human	WB,IHC	Cell Death Dis; HCRP-1 regulates EGFR-AKT-BIM-mediated anoikis resistance and serves as a prognostic marker in human colon cancer.; Authors - Feifei Chen ; KD Validated
human	WB	J Cell Sci; The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex.; Authors - Lydia Wunderley
human	WB	J Med Genet; A founder mutation in Vps37A causes autosomal recessive complex hereditary spastic paraparesis.; Authors - Zivony-Elboum Yifat Y