VPS37A Polyclonal antibody

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• KD/KO Validated

VPS37A Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 11870-1-AP Copied!

Publications (19)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

Vacuolar protein sorting-associated protein 37A, hVps37A, Hepatocellular carcinoma-related protein 1, HCRP1, ESCRT-I complex subunit VPS37A

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  WB analysis using 11870-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 11870-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 11870-1-AP

  WB result of VPS37A antibody (11870-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS37A transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 11870-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 11870-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 11870-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 11870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 11870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human liver using 11870-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VPS37A antibody (11870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, human liver tissue, MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11870-1-AP targets VPS37A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2453

  Product name: Recombinant human VPS37A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-174 aa of BC022363

  Sequence: MPDVPDAFPELSELSVSQLTDMNEQEEVLLEQFLTLPQLKQIITDKDDLVKSIEELARKNLLLEPSLEAKRQTVLDKYELLTQMKSTFEKKMQRQHELSESCSASALQARLKVAAHEAEEESDNIAEDFLEGKMEIDDFLSSFMEKRTICHCRRAKEEKLQQAIAMHSQFHAPL

• Full Name: vacuolar protein sorting 37 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC022363
• Gene Symbol: VPS37A
• Gene ID (NCBI): 137492
• RRID: AB_2215230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEZ2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

Publications

What published studies show

Anti-VPS37A (Cat# 11870-1-AP) has 19 citations published in journals including Cell Metabolism, Current Biology, Developmental Cell, Journal of Cell Biology, Autophagy, Cell Death & Disease, Cell Reports, Structure, iScience, and Oncotarget. Research fields span ESCRT-mediated endosomal sorting, autophagy, cancer biology (colorectal, prostate, renal, gastric), neurodegeneration (tau aggregate clearance), metabolic regulation, and hereditary spastic paraparesis.

Automatically generated from the 19 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Metab; Vps37a regulates hepatic glucose production by controlling glucagon receptor localization to endosomes; Authors - Revathi Sekar
human	WB,IF	Autophagy; Removal of hypersignaling endosomes by simaphagy; Authors - Simona M Migliano ; KO Validated
human	WB	Cell Death Dis; Inactivation of necroptosis-promoting protein MLKL creates a therapeutic vulnerability in colorectal cancer cells; Authors - Peijia Jiang
human	WB,IHC	Cell Death Dis; HCRP-1 regulates EGFR-AKT-BIM-mediated anoikis resistance and serves as a prognostic marker in human colon cancer.; Authors - Feifei Chen ; KD Validated
human	WB	Int J Biol Sci; HCRP-1 regulates cell migration, invasion and angiogenesis via Src/ FAK signaling in human prostate cancer.; Authors - Feifei Chen ; KD Validated
human	WB,IP,IF	Dev Cell; Transferrin receptor controls both autophagosome formation and closure via phosphatidylinositol 3-phosphate synthesis; Authors - Claudia Puri