VPS37C Polyclonal antibody

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  WB analysis using 27128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27128-1-AP (VPS37C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 27128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27128-1-AP (VPS37C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 27128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 27128-1-AP (VPS37C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 27128-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using VPS37C antibody (27128-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

VPS37C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27128-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ESCRT I complex subunit VPS37C, ESCRT-I complex subunit VPS37C, hVps37C, PML39, Vacuolar protein sorting-associated protein 37C

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Tested Applications

• Positive WB detected in: HT-29 cells, SK-BR-3 cells, T-47D cells
• Positive IHC detected in: mouse colon tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27128-1-AP targets VPS37C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26024

  Product name: Recombinant human VPS37C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-117 aa of BC141827

  Sequence: METLKDKTLQELEELQNDSEAIDQLALESPEVQDLQLEREMALATNRSLAERNLEFQGPLEISRSNLSDRYQELRKLVERCQEQKAKLEKFSSALQPGTLLDLLQVEGMKIEEESEA

• Full Name: vacuolar protein sorting 37 homolog C (S. cerevisiae)
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC141827
• Gene Symbol: VPS37C
• Gene ID (NCBI): 55048
• RRID: AB_3085928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A5D8V6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS37C (Vacuolar protein sorting-associated protein 37C) is also named as hVps37C, PML39 and ESCRT-I complex subunit VPS37C. VPS37C is a core subunit of the Endosomal sorting complex required for transport-I (ESCRT-I) complex (PMID: 37307706). VPS37C interacts with EKLF and prevents K48-linked polyubiquitination of EKLF and proteasome-mediated EKLF degradation, thus enhancing EKLF protein stability and transcriptional activity (PMID: 37307706).