VPS39 Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS39 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 16219-1-AP

Synonyms

hVam6p, KIAA0770, TLP, VAM6, Vam6/Vps39 like protein, VPS39

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Validation Data Gallery

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  IP experiment of mouse brain using 16219-1-AP

  IP result of anti-VPS39 (IP:16219-1-AP, 4ug; Detection:16219-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human small intestine using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human heart tissue, human small intestine tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Product Information

16219-1-AP targets VPS39 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS39 fusion protein Ag9267
• Full Name: vacuolar protein sorting 39 homolog (S. cerevisiae)
• Calculated Molecular Weight: 886 aa, 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC015817
• Gene Symbol: VPS39
• Gene ID (NCBI): 23339
• RRID: AB_11043179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS39, also named as KIAA0770, TLP, or VAM6, is a 886 amino acid protein, which contains one CNH domain and one CHCR repeat. VPS39 belongs to the VAM6/VPS39 family and localizes in the cytoplasm. VPS39 is widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen. VPS39 may play a role in clustering and fusion of late endosomes and lysosomes. VPS39 modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway.

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
	IP	Cell Host Microbe; Global mapping of Salmonella enterica-host protein-protein interactions during infection.; Authors - Philipp Walch
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	IF,WB	Dev Cell; ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress; Authors - Di Chen ; KD Validated
human	WB	Curr Biol; Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.; Authors - Anna Ulbricht
human,mouse	WB,IF	Mol Metab; SGPL1 stimulates VPS39 recruitment to the mitochondria in MICU1 deficient cells.; Authors - Joshua Jackson