VPS45 Polyclonal antibody

VPS45 Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 12006-1-AP

Synonyms

h VPS45, H1, H1VPS45, hlVps45, VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A

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Validation Data Gallery

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  WB analysis of RAW264.7 using 12006-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 12006-1-AP (VPS45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12006-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12006-1-AP (VPS45 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12006-1-AP

  Immunofluorescent analysis of HepG2 cells, using VPS45 antibody 12006-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

12006-1-AP targets VPS45 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS45 fusion protein Ag2658
• Full Name: vacuolar protein sorting 45 homolog (S. cerevisiae)
• Calculated molecular weight: 570 aa, 65 kDa
• GenBank accession number: BC012932
• Gene symbol: VPS45
• Gene ID (NCBI): 11311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

Publications

Species	Application	Title
human	WB	J Cell Sci; The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.; Authors - Laufman Orly O
		Gene; Rice protein suppresses ROS generation and stimulates antioxidant gene expression via Nrf2 activation in adult rats.; Authors - Hui Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jerome (Verified Customer) (05-28-2020)

Very inconclusive, a lot of background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 100
• Cell Tissue Type: HeLa

VPS45 Antibody Immunofluorescence validation (100 dilution) in HeLa (Cat no:12006-1-AP)