Tested Applications
| Positive WB detected in | mouse liver tissue, human kidney tissue, mouse brain tissue, rat pancreas tissue, rat brain tissue, rat liver tissue |
| Positive IP detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12616-1-AP targets VPS45 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3289 Product name: Recombinant human VPS45 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 271-570 aa of BC028382 Sequence: FYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEVLASGLHSRSKESSQVTSRSASRR Predict reactive species |
| Full Name | vacuolar protein sorting 45 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 570 aa, 65 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC028382 |
| Gene Symbol | VPS45 |
| Gene ID (NCBI) | 11311 |
| RRID | AB_2215367 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRW7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for VPS45 antibody 12616-1-AP | Download protocol |
| WB protocol for VPS45 antibody 12616-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VPS45 Polyclonal antibody (Cat# 12616-1-AP) has 5 citations. These appear in J Gastroenterol, Scientific Reports, Journal of Cell Science, Molecular Carcinogenesis, and Nature Communications. The associated research fields include hepatocellular carcinoma progression (via β1 integrin recycling and Wnt/β-catenin signaling), exosome-related gene diagnosis in NAFLD-associated HCC, Golgi COG complex–SNARE interactions, and metastasis suppressor identification in triple-negative breast cancer.
| Species | Application | Title |
|---|---|---|
J Gastroenterol VPS45 promotes the progression of hepatocellular carcinoma by recycling β1 integrin to the cell membrane via the endocytic pathway.
| ||
Sci Rep Predictive effect and clinical diagnosis significance of exosome-related genes for nonalcoholic fatty liver disease-related hepatocellular carcinoma. | ||
J Cell Sci The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes. | ||
Mol Carcinog VPS45 Contributes to the Progression of Hepatocellular Carcinoma by Triggering the Wnt/β-Catenin Signaling Pathway | ||
Nat Commun In vivo CRISPR screening identifies metastasis suppressors in triple-negative breast cancer. |
