VPS4B Monoclonal antibody, PBS Only

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  WB analysis using 68443-1-Ig (same clone as 68443-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 68443-1-Ig (same clone as 68443-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

  IP result of anti-VPS4B (IP:68443-1-Ig, 4ug; Detection:68443-1-Ig 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VPS4B antibody (68443-1-Ig, Clone: 2H3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

VPS4B Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 68443-1-PBS

Clone No.2H3G7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

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Product Information

68443-1-PBS targets VPS4B in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

• Tested Reactivity: Human, mouse , rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11610

  Product name: Recombinant human VPS4B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-444 aa of BC039574

  Sequence: MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG

• Full Name: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 444 aa, 49 kDa
• Observed Molecular Weight: ~50 kDa
• GenBank Accession Number: BC039574
• Gene Symbol: VPS4B
• Gene ID (NCBI): 9525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75351
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)