VPS53 Polyclonal antibody

VPS53 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12824-1-AP

Synonyms

Vacuolar protein sorting-associated protein 53 homolog, PP13624

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 12824-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12824-1-AP (VPS53 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12824-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12824-1-AP (VPS53 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12824-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12824-1-AP (VPS53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12824-1-AP

  IP result of anti-VPS53 (IP:12824-1-AP, 4ug; Detection:12824-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human stomach using 12824-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12824-1-AP (VPS53 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, L02 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12824-1-AP targets VPS53 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS53 fusion protein Ag3518
• Full Name: vacuolar protein sorting 53 homolog (S. cerevisiae)
• Calculated Molecular Weight: 832 aa, 94 kDa
• Observed Molecular Weight: 95-97 kDa
• GenBank Accession Number: BC029560
• Gene Symbol: VPS53
• Gene ID (NCBI): 55275
• RRID: AB_2215514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

Publications

Species	Application	Title
human	WB	Brain; Biallelic variants in VPS50 cause a neurodevelopmental disorder with neonatal cholestasis.; Authors - Pauline E Schneeberger