VPS72 Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS72 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15143-1-AP

Synonyms

CFL1, Protein YL 1, Swc2, TCFL1, Transcription factor like 1, VPS72, YL 1, YL1

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Validation Data Gallery

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  WB analysis of HeLa using 15143-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15143-1-AP

  WB result of VPS72 antibody (15143-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS72 transfected A549 cells.

• 

  WB analysis of mouse skeletal muscle using 15143-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15143-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15143-1-AP (VPS72 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 15143-1-AP

  IP result of anti-VPS72 (IP:15143-1-AP, 4ug; Detection:15143-1-AP 1:500) with NIH/3T3 cells lysate 4000 ug.

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  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 15143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 15143-1-AP (VPS72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, NIH/3T3 cells, A549 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human breast hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15143-1-AP targets VPS72 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS72 fusion protein Ag7424
• Full Name: vacuolar protein sorting 72 homolog (S. cerevisiae)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC003151
• Gene Symbol: VPS72
• Gene ID (NCBI): 6944
• RRID: AB_2215534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian TRRAP/TIP60-containing histone acetyltransferase (HAT) complex, which exists in Drosophila melanogaster and mammalian cells, is a complex that is responsible for various cellular processes, including DNA repair, transcriptional activation and apoptosis. Vacuolar protein sorting-associated protein 72 homolog (VPS72), also known as VPS72 or Transcription factor-like 1, is a 364 amino acid subunit of the TRRAP/TIP60 HAT complex. VPS72 has also been identified as a subunit of a novel complex containing SNF2-related helicase SRCAP (SWI2/SNF2-related CBP activator protein). This SRCAP-containing complex is very similar to the S. cerevisiae SWR1 chromatin remodeling complex. The involvement of VPS72 in these complexes has suggested that VPS72 plays multiple roles in chromatin modification and remodeling in cells. VPS72 localizes to the nucleus and is phosphorylated upon DNA damage, most likely by ATM or ATR.

Publications

Species	Application	Title
		Mol Cell; PMID: 28366643; Authors - Sophie Barral
	WB	Cell Discov; Structural insights into histone exchange by human SRCAP complex; Authors - Jiali Yu