VRK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

VRK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 28018-1-AP

Synonyms

Vaccinia-related kinase 1, Serine/threonine-protein kinase VRK1, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 28018-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28018-1-AP (VRK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 28018-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 28018-1-AP (VRK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 28018-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28018-1-AP (VRK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human skin cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

28018-1-AP targets VRK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VRK1 fusion protein Ag27754
• Full Name: vaccinia related kinase 1
• Calculated Molecular Weight: 396 aa, 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC103761
• Gene Symbol: VRK1
• Gene ID (NCBI): 7443
• RRID: AB_2918140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vaccinia-related kinase 1 (VRK1) is a members of the vaccinia-related kinase (VRK) family of serine/threonine kinases and are principally localized in the nucleus. VRK1 was initially described as a kinase with homology to B1R, a vaccinia virus kinase required for viral DNA replication and the early phase of infection. It has been suggested that VRK1 was overexpressed in numerous human tumors, such as lung carcinomas, hepatocellular carcinoma, breast carcinomas, head and neck squamous cell carcinoma. (PMID: 28927264, PMID: 29103766)

Publications

Species	Application	Title
human,mouse	WB,IHC	Can Respir J; Downregulation of VRK1 Inhibits Progression of Lung Squamous Cell Carcinoma through DNA Damage; Authors - Ning Du ; KD Validated
human	IHC	J Inflamm Res; Identification of VRK1 as a Novel Potential Biomarker for Prognosis and Immunotherapy in Hepatocellular Carcinoma; Authors - Xiaoyan He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alejandro (Verified Customer) (07-02-2024)

It works nicely

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T and SF8628