VRK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

VRK2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12946-1-AP

Synonyms

vaccinia related kinase 2, VRK2

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Validation Data Gallery

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  WB analysis using 12946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12946-1-AP

  WB result of VRK2 antibody (12946-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VRK2 transfected HepG2 cells.

• 

  WB analysis of human liver using 12946-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12946-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12946-1-AP

  IP result of anti-VRK2 (IP:12946-1-AP, 4ug; Detection:12946-1-AP 1:500) with K-562 cells lysate 3600ug.

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue, mouse skeletal muscle tissue, K-562 cells, U2OS cells, BxPC-3 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

12946-1-AP targets VRK2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VRK2 fusion protein Ag4019
• Full Name: vaccinia related kinase 2
• Calculated Molecular Weight: 508 aa, 58 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC027854
• Gene Symbol: VRK2
• Gene ID (NCBI): 7444
• RRID: AB_2215681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	Int J Biol Sci; VRK2 activates TNFα/NF-κB signaling by phosphorylating IKKβ in pancreatic cancer.; Authors - Jionghuang Chen
mouse	WB	BMC Med; Reduced Vrk2 expression is associated with higher risk of depression in humans and mediates depressive-like behaviors in mice; Authors - Mei-Yu Yin ; KO Validated
human	WB,IHC	Cell Rep; Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic mice; Authors - Xiao-Tong Lin ; KD Validated