VSIG4 Polyclonal antibody

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• VSIG4 Polyclonal antibody (27829-1-AP)

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  WB analysis using 27829-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27829-1-AP (VSIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

VSIG4 Polyclonal Antibody for WB, ELISA

Cat No. 27829-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CRIg, Protein Z39Ig, UNQ317/PRO362, V-set and immunoglobulin domain-containing protein 4, Z39IG

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Tested Applications

• Positive WB detected in: TT cells, U-251 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27829-1-AP targets VSIG4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27214

  Product name: Recombinant human VSIG4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 305-399 aa of BC010525

  Sequence: MLCRKTSQQEHVYEAARAHAREANDSGETMRVAIFASGCSSDEPTSQNLGNNYSDEPCIGQEYQIIAQINGNYARLLDTVPLDYEFLATEGKSVC

• Full Name: V-set and immunoglobulin domain containing 4
• Calculated Molecular Weight: 399 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC010525
• Gene Symbol: VSIG4
• Gene ID (NCBI): 11326
• RRID: AB_3742260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y279
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

V-set and immunoglobulin domain-containing 4 (VSIG4) is a complement receptor of the immunoglobulin superfamily (CRIg) that is specifically expressed in resting tissue-resident macrophages . Notably, CRIg is expressed in monocyte-derived macrophages but not in human and mouse monocytes. VSIG4 can suppress T cell proliferation and promote Foxp3+ regulatory T cell (Tregs) differentiation by binding an unidentified receptor on T cells . VSIG4 has been implicated in a variety of diseases, particularly those involving immune dysregulation and inflammation, such as non-alcoholic steatohepatitis, rheumatoid arthritis, and obesity-related metabolic disorders . Recently, VSIG4+ tumor-associated macrophages (TAMs) have been identified as a specific subset that infiltrates aggressive cancers, including anaplastic thyroid cancer (ATC). (PMID: 37153746,PMID: 39920772)