VILIP-1 Polyclonal antibody

VILIP-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13919-1-AP

Synonyms

VILIP 1, VILIP1, VSNL1, VILIP, HUVISL1

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Validation Data Gallery

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  WB analysis using 13919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse tongue using 13919-1-AP

  mouse tongue tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13919-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 13919-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13919-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13919-1-AP

  IP result of anti-VILIP-1 (IP:13919-1-AP, 3ug; Detection:13919-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of Human bowens disease using 13919-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 13919-1-AP (VILIP-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13919-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VILIP-1 antibody (13919-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse tongue tissue, human brain tissue, rat brain tissue, HeLa cells, mouse cerebellum tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: Human bowens disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

13919-1-AP targets VILIP-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VILIP-1 fusion protein Ag4923
• Full Name: visinin-like 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22-24 kDa
• GenBank Accession Number: BC022012
• Gene Symbol: VSNL1
• Gene ID (NCBI): 7447
• RRID: AB_2215851
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human, mouse	WB,IF	Free Radic Biol Med; Dimerization of visinin-like-protein 1 is regulated by oxidative stress and calcium and is a pathological hallmark of amyotrophic lateral sclerosis.; Authors - Martina P Liebl
mouse	WB	Front Cell Neurosci; Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1.; Authors - Anna Sancho-Balsells