VSX1 Polyclonal antibody

VSX1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 23566-1-AP

Synonyms

Homeodomain protein RINX, KTCN, PPCD, PPD, RINX, Transcription factor VSX1, visual system homeobox 1, VSX1

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Validation Data Gallery

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  WB analysis of mouse eye using 23566-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 23566-1-AP (VSX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 23566-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23566-1-AP (VSX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse eye using 23566-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using 23566-1-AP (VSX1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse eye tissue, Raji cells
• Positive IF detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23566-1-AP is VSX1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VSX1 fusion protein Ag19222
• Full Name: visual system homeobox 1
• Calculated Molecular Weight: 365 aa, 38 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC126228
• Gene Symbol: VSX1
• Gene ID (NCBI): 30813
• RRID: AB_2879297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VSX1(visual system homeobox 1) is a member of the Vsx1 group of vertebrate paired-like homeodomain transcription factors locating to the nucleus. Mutations in the VSX1 gene in keratoconus have been reported in many studies. VSX1 is considered important in ocular development and is particularly involved in the developing cornea (PMID:21139977). And previous studies have identified that Vsx1 participates in regulating retinal progenitor proliferation, differentiation and functional maintenance of bipolar cells (PMID:25150888).It was demonstrated that Vsx1 express in embryonic craniofacial, adult corneal, and adult retinal cDNA libraries.VSX1 mRNA has been found in the outer tier of the inner nuclear layer of the human retina and the cornea (PMID: 21139977).