Tested Applications
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | human retinoblastoma tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
| Positive IF-Fro detected in | mouse eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25825-1-AP targets VSX2 in WB, IHC, IF-P, IF-Fro, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23035 Product name: Recombinant human VSX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC128153 Sequence: MTGKAGEALSKPKSETVAKSTSGGAPARCTGFGIQEILGLNKEPPSSHPRAALDGLAPGHLLAARSVLSPAGVGGMGLLGPGGLPGFYTQPTFLEVLSDPQSVHLQPLGRASGPLDTSQTASSDSEDVSSSDRKMSKSALNQTKKRK Predict reactive species |
| Full Name | visual system homeobox 2 |
| Calculated Molecular Weight | 361 aa, 39 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC128153 |
| Gene Symbol | VSX2 |
| Gene ID (NCBI) | 338917 |
| RRID | AB_2880257 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P58304 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VSX2 antibody 25825-1-AP | Download protocol |
| IHC protocol for VSX2 antibody 25825-1-AP | Download protocol |
| WB protocol for VSX2 antibody 25825-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VSX2 Polyclonal antibody (Cat# 25825-1-AP) has 5 citations published in Cell Stem Cell, Nature Protocols, Pharmacological Research, Cell Biosciences, and eLife. The associated research fields include brain and forebrain organoid development, retinal development, retinal neurodegeneration, and neuronal lineage specification in the anterior brainstem.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Human brain organoids assemble functionally integrated bilateral optic vesicles. | ||
Nat Protoc Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia | ||
Pharmacol Res NAALAD2 mutations disrupt the fate of photoreceptor cells and retinal pigment epithelial cells during early retinal development | ||
Cell Biosci NOTCH2NLC GGC repeat expansions cause retinal neurodegeneration in neuronal intranuclear inclusion disease mouse model. | ||
Elife Gene regulatory mechanisms guiding bifurcation of inhibitory and excitatory neuron lineages in the mouse anterior brainstem. |
Reviews
What customers say
One reviewer rated this product 5 stars after using it for immunofluorescence. They reported no unspecific staining and described the overall outcome as great, indicating strong specificity and reliable performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alessandro (Verified Customer) (11-06-2022) | no unspecific staining, great outcome
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