VSX2 Monoclonal antibody

VSX2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C7C1

Cat no : 67626-1-Ig

Synonyms

CHX10, Homeobox protein CHX10, HOX10, MCOP2, MCOPCB3, RET1, visual system homeobox 2, VSX2

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Validation Data Gallery

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  WB analysis using 67626-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67626-1-Ig (VSX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of rat eye using 67626-1-Ig

  rat eye tissue were subjected to SDS PAGE followed by western blot with 67626-1-Ig (VSX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, rat eye tissue, Ramos cells, Daudi cells, NCCIT cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67626-1-Ig targets VSX2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: VSX2 fusion protein Ag29867
• Full Name: visual system homeobox 2
• Calculated Molecular Weight: 361 aa, 39 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC128153
• Gene Symbol: VSX2
• Gene ID (NCBI): 338917
• RRID: AB_2882827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VSX2, also named as CHX10 and HOX10, belongs to the paired homeobox family. VSX2 plays a significant role in the specification and morphogenesis of the sensory retina. It may also participate in the development of the cells of the inner nuclear layer, particularly bipolar cells. Defects in VSX2 are the cause of microphthalmia isolated type 2 (MCOP2). Defects in VSX2 are the cause of microphthalmia with cataracts and iris abnormalities (MCOPCTI). Defects in VSX2 are the cause of microphthalmia isolated with coloboma type 3 (MCOPCB3). The antibody is specific to VSX2.