VTI1A Polyclonal antibody

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• VTI1A Polyclonal antibody (12354-1-AP)

VTI1A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 12354-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Vesicle transport through interaction with t-SNAREs homolog 1A, Vesicle transport v-SNARE protein Vti1-like 2, Vti1-rp2

• 

  WB analysis of human brain using 12354-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 12354-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human lung using 12354-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 12354-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human kidney using 12354-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 12354-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MCF-7 using 12354-1-AP

  IP result of anti-VTI1A (IP:12354-1-AP, 3ug; Detection:12354-1-AP 1:1000) with MCF-7 cells lysate 520ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 12354-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 12354-1-AP (VTI1A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human brain tissue, human heart tissue, human kidney tissue, human lung tissue, MCF-7 cells, mouse brain tissue
• Positive IP detected in: MCF-7 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12354-1-AP targets VTI1A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3018

  Product name: Recombinant human VTI1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-203 aa of BC017052

  Sequence: MSSDFEGYEQDFAVLTAEITSKIARVPRLPPDEKKQMVANVEKQLEEAKELLEQMDLEVREIPPQSRGMYSNRMRSYKQEMGKLETDFKRSRIAYSDEVRNELLGDDGNSSENQRAHLLDNTERLERSSRRLEAGYQIAVETEQIGQEMLENLSHDREKIQRARERLRETDANLGKSSRILTGMLRRGCSVKKQCNLSLAPKA

• Full Name: vesicle transport through interaction with t-SNAREs homolog 1A (yeast)
• Calculated Molecular Weight: 217 aa, 25 kDa
• Observed Molecular Weight: 25-29 kDa
• GenBank Accession Number: BC017052
• Gene Symbol: VTI1A
• Gene ID (NCBI): 143187
• RRID: AB_2216150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96AJ9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). VTI1A is involved in transport between the endosome and the trans-Golgi network. The SNARE complex of STX10, STX16, VTI1A, and VAMP3 is required for MPR (mannose 6-phosphate receptors) transport from endosomes to the Golgi after delivering lysosomal enzymes to the endocytic pathway (PMID: 18195106). Transport from early/recycling endosomes to the TGN is mediated by the SNARE complex of STX6, STX16, VTI1A and VAMP4 (PMID: 11839770; 21807881).

Publications

What published studies show

The VTI1A Polyclonal antibody (Cat# 12354-1-AP) has 10 citations published in journals including Cell, Molecular Psychiatry, PNAS, Oncogene, Journal of Cell Biology, Cells, Oncotarget, Journal of Cell Science, Biochemical Journal, and Traffic. Research fields span vesicle trafficking, neuronal activity in autism, Alzheimer's disease, hepatocellular carcinoma, endosome-to-TGN transport, Fabry disease, ovarian cancer, Golgi SNARE complex assembly, and phosphoproteomics of endosomal proteins.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
mouse	WB	Mol Psychiatry; Cadherin 11 regulates presynaptic vesicle trafficking and neuronal activity in autism-related brain circuit.; Authors - Xiaoxiao Cao
mouse	WB	Proc Natl Acad Sci U S A; Nanoelectrochemistry reveals how soluble Aβ42 oligomers alter vesicular storage and release of glutamate; Authors - Xiao-Ke Yang
human	WB,IF	Oncogene; Syntaxin-6 mediated autophagy confers lenvatinib resistance in hepatocellular carcinoma; Authors - Guo-Pei Zhang
human	WB,IF	J Cell Biol; The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport.; Authors - Laufman Orly O
human	WB	Cells; Generation of GLA-Knockout Human Embryonic Stem Cell Lines to Model Autophagic Dysfunction and Exosome Secretion in Fabry Disease-Associated Hypertrophic Cardiomyopathy.; Authors - Hui-Yung Song