VWA1 Polyclonal antibody

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• VWA1 Polyclonal antibody (14322-1-AP)

VWA1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14322-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

VWA 1, VWA1, WARP

• 

  WB analysis of T-47D using 14322-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 14322-1-AP (VWA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 14322-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 14322-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 14322-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14322-1-AP (VWA1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 14322-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14322-1-AP (VWA1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: T-47D cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14322-1-AP targets VWA1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5565

  Product name: Recombinant human VWA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-445 aa of BC059409

  Sequence: MLPWTALGLALSLRLALARSGAERGPPASAPRGDLMFLLDSSASVSHYEFSRVREFVGQLVAPLPLGTGALRASLVHVGSRPYTEFPFGQHSSGEAAQDAVRASAQRMGDTHTGLALVYAKEQLFAEASGARPGVPKVLVWVTDGGSSDPVGPPMQELKDLGVTVFIVSTGRGNFLELSAAASAPAEKHLHFVDVDDLHIIVQELRGSILDAMRPQQLHATEITSSGFRLAWPPLLTADSGYYVLELVPSAQPGAARRQQLPGNATDWIWAGLDPDTDYDVALVPESNVRLLRPQILRVRTRPGEAGPGASGPESGAGPAPTQLAALPAPEEAGPERIVISHARPRSLRVSWAPALGSAAALGYHVQFGPLRGGEAQRVEVPAGRNCTTLQGLAPGTAYLVTVTAAFRSGRESALSAKACTPDGPRPRPRPVPRAPTPGTASREP

• Full Name: von Willebrand factor A domain containing 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC059409
• Gene Symbol: VWA1
• Gene ID (NCBI): 64856
• RRID: AB_2241694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6PCB0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VWA1 (also known as WARP) is a member of the von Willebrand factor A-domain (VA-domain) superfamily of extracellular matrix proteins which may play a role in cartilage structure and function (PMID: 12062410). VWA1 is a multimeric component of the chondrocyte pericellular matrix in articular cartilage and intervertebral disc, where it interacts with the basement membrane heparan sulfate proteoglycan perlecan (PMID: 18314316). VWA1 may have a role in maintaining the blood-brain barrier.

Publications

What published studies show

The VWA1 Polyclonal antibody (Cat# 14322-1-AP) has 3 citations. These appear in Nature Communications, Theranostics, and Clinical and Translational Oncology. The associated research fields include pancreatic islet biology and diabetes, hepatocellular carcinoma, and Kaposi's sarcoma. Applications cited include IHC, WB, and IF, all in human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Nat Commun; Single-cell atlas of human pancreatic islet and acinar endothelial cells in health and diabetes; Authors - Rebecca Craig-Schapiro
human	WB	Theranostics; Survey of the translation shifts in hepatocellular carcinoma with ribosome profiling.; Authors - Qin Zou
human	IHC,IF	Clin Transl Oncol; Single-cell and spatial transcriptomics reveal mTOR-driven cellular fate of spindle cells and immune evasion in classic Kaposi's sarcoma.; Authors - Ting Su