VWA1 Polyclonal antibody

VWA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14322-1-AP

Synonyms

VWA 1, VWA1, WARP

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Validation Data Gallery

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  WB analysis of T-47D using 14322-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 14322-1-AP (VWA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14322-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14322-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14322-1-AP (VWA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14322-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14322-1-AP (VWA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14322-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14322-1-AP (VWA1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: T-47D cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14322-1-AP targets VWA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VWA1 fusion protein Ag5565
• Full Name: von Willebrand factor A domain containing 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC059409
• Gene Symbol: VWA1
• Gene ID (NCBI): 64856
• RRID: AB_2241694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VWA1 (also known as WARP) is a member of the von Willebrand factor A-domain (VA-domain) superfamily of extracellular matrix proteins which may play a role in cartilage structure and function (PMID: 12062410). VWA1 is a multimeric component of the chondrocyte pericellular matrix in articular cartilage and intervertebral disc, where it interacts with the basement membrane heparan sulfate proteoglycan perlecan (PMID: 18314316). VWA1 may have a role in maintaining the blood-brain barrier.

Publications

Species	Application	Title
human	WB	Theranostics; Survey of the translation shifts in hepatocellular carcinoma with ribosome profiling.; Authors - Qin Zou