VWA2 Polyclonal antibody

VWA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18714-1-AP

Synonyms

von Willebrand factor A domain-containing protein 2, NET42, CCSP 2, AMACO, A domain-containing protein similar to matrilin and collagen

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Validation Data Gallery

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  WB analysis of A375 using 18714-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18714-1-AP (VWA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 18714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18714-1-AP (VWA2 Antibody) at dilution of 1:50.

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  IHC staining of human colon using 18714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18714-1-AP (VWA2 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 18714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 18714-1-AP (VWA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 18714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 18714-1-AP (VWA2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 18714-1-AP

  Immunofluorescent analysis of A375 cells using 18714-1-AP (VWA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 18714-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using VWA2 antibody (18714-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: A375 cells
• Positive IHC detected in: human colon tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells, A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18714-1-AP targets VWA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: von Willebrand factor A domain containing 2
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC128588
• Gene Symbol: VWA2
• Gene ID (NCBI): 340706
• RRID: AB_10598772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VWA2, also named as AMACO and CCSP2, is a novel candidate serological marker of colon neoplasia. VWA2 is found in the media of variesy of established cell lines of both fibroblast and epithelial origin. This antibody can recognize all the 3 isoforms of VWA2.