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VWA8 Polyclonal antibody, PBS Only (31348-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 31348-1-AP (same clone as 31348-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 31348-1-AP (KIAA0564 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31348-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 31348-1-AP (same clone as 31348-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31348-1-AP (VWA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31348-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 31348-1-AP (same clone as 31348-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31348-1-AP (VWA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31348-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 31348-1-AP (same clone as 31348-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using VWA8 antibody (31348-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31348-1-PBS in a different storage buffer formulation.

VWA8 Polyclonal antibody, PBS Only

Name: VWA8 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 31348-1-PBS

VWA8 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 31348-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RP97, P7BP2, KIAA0564

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

31348-1-PBS targets VWA8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag35404 Product name: Recombinant human KIAA0564 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1700-1905 aa of NM_015058.1 Sequence: ELEPQLGSPQQKPKRLRLVVDVSGSMYRFNRMDGRLERTMEAVCMVMEAFENYEEKFQYDIVGHSGDGYNIGLVPMNKIPKDNKQRLEILKTMHAHSQFCMSGDHTLEGTEHAIKEIVKEEADEYFVIVLSDANLSRYGIHPAKFAQILTRDPQVNAFAIFIGSLGDQATRLQRTLPAGRSFVAMDTKDIPQILQQIFTSTMLSSV Predict reactive species
Full Name	KIAA0564
Calculated Molecular Weight	215kDa，1905aa
Observed Molecular Weight	204-215 kDa, 117 kDa
GenBank Accession Number	NM_015058.1
Gene Symbol	KIAA0564
Gene ID (NCBI)	23078
RRID	AB_3669955
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	A3KMH1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

VWA8, also known as KIAA0564, is a mitochondrial matrix-targeted protein having a putative ATPase activity (PMID: 34660594). It has 3 isoforms with the MW of 215, 204, and 117 kDa.