Vegfa Polyclonal antibody

Vegfa Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26157-1-AP

Synonyms

Vegf, Vegf a, Vegf120, Vegf164, Vegf188, Vegfa, Vpf



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 26157-1-AP is Vegfa Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vegfa fusion protein Ag24084
Full Name vascular endothelial growth factor A
Observed molecular weight 45 kDa
GenBank accession numberNM-001287056
Gene symbol Vegfa
Gene ID (NCBI) 22339
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

Protocols

Product Specific Protocols
WB protocol for Vegfa antibody 26157-1-APDownload protocol
IHC protocol for Vegfa antibody 26157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice.

Authors - Dongdong Huang
ratWB

Respir Res

The role of RNA m6A methylation in the regulation of postnatal hypoxia-induced pulmonary hypertension.

Authors - Shanshan Xu
mouseWB

Biochem Biophys Res Commun

Ablation of glutaredoxin 1 promotes pulmonary angiogenesis and alveolar formation in hyperoxia-injured lungs by modifying HIF-1α stability and inhibiting the NF-κB pathway.

Authors - Xuwei Liu